Hotspot

trnS-GCU-trnG-UCC

LSC · 7830-8561 · 732 bp

Back to Veronica

Pi 0.0684

MarkerSeek score 77.10

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 732
variable_sites: 188
pi: 0.0684
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	598-832 bp	1.0	63.7
trnS-GCU-trnG-UCC_p2	AATCCGACGCTTTAGTCCAC	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	597-831 bp	1.0	63.7
trnS-GCU-trnG-UCC_p3	ACGGAAAGAGAGGGATTCGA	ACGAATCACACTTTTACCACT	50.0	38.1	58.2	55.5	652-886 bp	1.0	62.5
trnS-GCU-trnG-UCC_p4	CAATCCGACGCTTTAGTCCA	AACGAATCACACTTTTACCACT	50.0	36.4	58.0	56.2	599-833 bp	1.0	61.8
trnS-GCU-trnG-UCC_p5	AATCCGACGCTTTAGTCCAC	AACGAATCACACTTTTACCACT	50.0	36.4	58.0	56.2	598-832 bp	1.0	61.7