Hotspot

trnK-UUU-rps16

LSC · 4213-4792 · 580 bp

Back to Veronica

Pi 0.0872

MarkerSeek score 78.50

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 580
variable_sites: 179
pi: 0.0872
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	GCTCTTCCCTTATCAGGCAC	TCTATCCCAATGAGCCGTCT	55.0	50.0	58.0	57.9	1152-1432 bp	1.0	91.0
trnK-UUU-rps16_p2	GGCCAATGGTATGGACGAAT	TCTATCCCAATGAGCCGTCT	50.0	50.0	58.0	57.9	862-1142 bp	0.96	89.5
trnK-UUU-rps16_p3	GCTCTTCCCTTATCAGGCAC	AAAAAGGCGCTCAACCTACA	55.0	45.0	58.0	58.0	1046-1097 bp	0.4	64.8
trnK-UUU-rps16_p4	AGCTCTTCCCTTATCAGGCA	AAAAAGGCGCTCAACCTACA	50.0	45.0	58.1	58.0	1047-1098 bp	0.4	64.2
trnK-UUU-rps16_p5	GGCCAATGGTATGGACGAAT	AAAAAGGCGCTCAACCTACA	50.0	45.0	58.0	58.0	757-808 bp	0.36	64.2