Hotspot

ycf1

SSC · 122528-127573 · 5046 bp

Back to Verbesina

Pi 0.00972

MarkerSeek score 43.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5046
variable_sites: 49
pi: 0.00972
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGTTGTAGAGCGATTCTGCG	TCACAGGCAGAAGAAGAAATGA	50.0	40.9	58.1	57.6	233-236 bp	1.0	63.9
ycf1_p2	AGTTGTAGAGCGATTCTGCG	CACAGGCAGAAGAAGAAATGA	50.0	42.9	58.1	56.2	232-235 bp	1.0	60.1
ycf1_p3	AGTTGTAGAGCGATTCTGCG	TCACAGGCAGAAGAAGAAATG	50.0	42.9	58.1	56.2	233-236 bp	1.0	60.1
ycf1_p4	AGTTGTAGAGCGATTCTGCG	CACAGGCAGAAGAAGAAATGAA	50.0	40.9	58.1	56.9	232-235 bp	1.0	57.9
ycf1_p5	AGTTGTAGAGCGATTCTGCG	TTCACAGGCAGAAGAAGAAATG	50.0	40.9	58.1	56.9	234-237 bp	1.0	57.9