Hotspot

rpoA

LSC · 77534-78547 · 1014 bp

Back to Verbesina

Pi 0.00592

MarkerSeek score 48.40

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 1014
variable_sites: 6
pi: 0.00592
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	ACCGATTTTGGCCCTTTTCT	GGCTTCCGAGGTACAAGAAG	45.0	55.0	58.0	58.0	1400 bp	1.0	80.6
rpoA_p2	ACCGATTTTGGCCCTTTTCT	TACTTGTGGCTTCCGAGGTA	45.0	50.0	58.0	58.1	1407 bp	1.0	80.1
rpoA_p3	CTCGAGCCGGATGATGAAAA	GGCTTCCGAGGTACAAGAAG	50.0	55.0	58.1	58.0	2096 bp	1.0	79.9
rpoA_p4	ACCGATTTTGGCCCTTTTCT	GCTTCCGAGGTACAAGAAGG	45.0	55.0	58.0	58.0	1399 bp	0.5	59.8
rpoA_p5	CTCGAGCCGGATGATGAAAA	GCTTCCGAGGTACAAGAAGG	50.0	55.0	58.1	58.0	2095 bp	0.5	59.4