Hotspot

ndhF

SSC · 108804-111032 · 2229 bp

Back to Verbesina

Pi 0.00674

MarkerSeek score 46.30

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2229
variable_sites: 15
pi: 0.00674
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GCCGAGAAGAAGGTATCAGC	GAGGTAAGATCCGGTGAATCG	55.0	52.4	58.1	58.0	2700-2713 bp	1.0	71.7
ndhF_p2	CTAGCACTGGGTAGACCTCA	GAGGTAAGATCCGGTGAATCG	55.0	52.4	57.9	58.0	2616-2629 bp	1.0	71.7
ndhF_p3	TCGTTGTGGTCGGACTCTAT	GAGGTAAGATCCGGTGAATCG	50.0	52.4	58.2	58.0	2804-2817 bp	1.0	71.4
ndhF_p4	ATCAATTCGGTCGTTGTGGT	GAGGTAAGATCCGGTGAATCG	45.0	52.4	57.8	58.0	2814-2827 bp	1.0	71.4
ndhF_p5	AGAAGAAGGAGCCGAGAAGA	GAGGTAAGATCCGGTGAATCG	50.0	52.4	57.8	58.0	2710-2723 bp	1.0	71.0