Hotspot

ndhF-rpl32

SSC · 111033-112036 · 1004 bp

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Pi 0.014

MarkerSeek score 64.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1004
variable_sites: 14
pi: 0.014
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTTGCCGTCGGAAAAAGAAG	CGCCCCATATCCTTTCCTTT	50.0	50.0	58.0	57.6	1183-1242 bp	1.0	78.1
ndhF-rpl32_p2	TGCCGTCGGAAAAAGAAGAA	CGCCCCATATCCTTTCCTTT	45.0	50.0	58.0	57.6	1181-1240 bp	1.0	78.0
ndhF-rpl32_p3	TTGCCGTCGGAAAAAGAAGA	CGCCCCATATCCTTTCCTTT	45.0	50.0	58.0	57.6	1182-1241 bp	1.0	78.0
ndhF-rpl32_p4	GTTGCCGTCGGAAAAAGAAG	TGACAAAGCTTTTAACGCCG	50.0	45.0	58.0	57.6	1201-1260 bp	1.0	77.8
ndhF-rpl32_p5	TGCCGTCGGAAAAAGAAGAA	TGACAAAGCTTTTAACGCCG	45.0	45.0	58.0	57.6	1199-1258 bp	1.0	77.7