Hotspot

ycf1-ndhF

SSC · 108790-108803 · 14 bp

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Pi 0.143

MarkerSeek score 73.20

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 14
variable_sites: 2
pi: 0.143
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	CTAGCACTGGGTAGACCTCA	GGGGGTGGACGTATTTCTTC	55.0	55.0	57.9	58.0	424-437 bp	1.0	82.8
ycf1-ndhF_p2	GCCGAGAAGAAGGTATCAGC	GGGGGTGGACGTATTTCTTC	55.0	55.0	58.1	58.0	508-521 bp	1.0	82.2
ycf1-ndhF_p3	CTAGCACTGGGTAGACCTCA	TGTGGGGGTGAGAAAATTGG	55.0	50.0	57.9	58.0	542-555 bp	1.0	82.1
ycf1-ndhF_p4	GCCGAGAAGAAGGTATCAGC	TGTGGGGGTGAGAAAATTGG	55.0	50.0	58.1	58.0	626-639 bp	1.0	81.7
ycf1-ndhF_p5	TCGTTGTGGTCGGACTCTAT	TGTGGGGGTGAGAAAATTGG	50.0	50.0	58.2	58.0	730-743 bp	1.0	81.1