Hotspot

trnW-CCA-trnP-UGG

LSC · 66182-66343 · 162 bp

Back to Verbesina

Pi 0.0185

MarkerSeek score 63.10

Primer available yes

Label trnW-CCA-trnP-UGG

Feature Metadata

label_name: trnW-CCA-trnP-UGG
feature_type: igs
region: LSC
length_bp: 162
variable_sites: 3
pi: 0.0185
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnW-CCA-trnP-UGG_p1	GCGTTCTACCGAACTGAACT	GGGTACAAAATGTCACGGGT	50.0	50.0	57.9	58.1	229 bp	1.0	79.8
trnW-CCA-trnP-UGG_p2	GCGTTCTACCGAACTGAACT	TGGGTACAAAATGTCACGGG	50.0	50.0	57.9	58.1	230 bp	1.0	79.7
trnW-CCA-trnP-UGG_p3	GCGTTCTACCGAACTGAACT	GTCACGGGTTCAAATCCTGT	50.0	50.0	57.9	58.1	218 bp	1.0	79.7
trnW-CCA-trnP-UGG_p4	GCGTTCTACCGAACTGAACT	CACGGGTTCAAATCCTGTCA	50.0	50.0	57.9	58.1	216 bp	1.0	79.7
trnW-CCA-trnP-UGG_p5	GCGTTCTACCGAACTGAACT	TGTCACGGGTTCAAATCCTG	50.0	50.0	57.9	58.1	219 bp	1.0	79.7