Hotspot

psbE-petL

LSC · 64313-65609 · 1297 bp

Back to Verbesina

Pi 0.0093

MarkerSeek score 64.50

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1297
variable_sites: 12
pi: 0.0093
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.9	57.1	1484-1489 bp	1.0	73.0
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.8	57.1	1407-1412 bp	1.0	72.2
psbE-petL_p3	ACGAATAACCAACCCGCAAT	GTCGTATCTTGCTCAGACCA	45.0	50.0	58.2	57.1	1493-1498 bp	1.0	72.1
psbE-petL_p4	TGCTGACGAATAACCAACCC	GTCGTATCTTGCTCAGACCA	50.0	50.0	58.2	57.1	1498-1503 bp	1.0	72.0
psbE-petL_p5	CGAATAACCAACCCGCAATG	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.8	57.1	1492-1497 bp	1.0	71.9