Hotspot

atpA-trnR-UCU

LSC · 30060-30187 · 128 bp

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Pi 0.0391

MarkerSeek score 73.80

Primer available yes

Label atpA-trnR-UCU

Feature Metadata

label_name: atpA-trnR-UCU
feature_type: igs
region: LSC
length_bp: 128
variable_sites: 5
pi: 0.0391
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-trnR-UCU_p1	TACTTCAGGAACAAGCAGCC	GGTTCAAATCCTATTGGACGC	50.0	47.6	57.8	57.3	173-177 bp	1.0	67.3
atpA-trnR-UCU_p2	CAGGAACAGAGGGAACGTTT	GGTTCAAATCCTATTGGACGC	50.0	47.6	57.7	57.3	195-199 bp	1.0	66.4
atpA-trnR-UCU_p3	TACTTCAGGAACAAGCAGCC	CAATGAAAGCGTCCATTGTCT	50.0	42.9	57.8	57.2	231-235 bp	1.0	65.5
atpA-trnR-UCU_p4	CTTCAGGAACAAGCAGCCTA	GGTTCAAATCCTATTGGACGC	50.0	47.6	57.5	57.3	171-175 bp	1.0	64.9
atpA-trnR-UCU_p5	CAGGAACAGAGGGAACGTTT	CAATGAAAGCGTCCATTGTCT	50.0	42.9	57.7	57.2	253-257 bp	1.0	64.8