Hotspot

atpF-atpA

LSC · 28460-28532 · 73 bp

Back to Verbesina

Pi 0.0145

MarkerSeek score 65.40

Primer available yes

Label atpF-atpA

Feature Metadata

label_name: atpF-atpA
feature_type: igs
region: LSC
length_bp: 73
variable_sites: 1
pi: 0.0145
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpA_p1	CATATTGGCGGCGATGAAAC	GTCGGCTTGAATGGTTACCA	50.0	50.0	58.0	58.2	124-128 bp	1.0	81.4
atpF-atpA_p2	CATATTGGCGGCGATGAAAC	ATTTCGTCGGCTTGAATGGT	50.0	45.0	58.0	58.2	129-133 bp	1.0	81.3
atpF-atpA_p3	CATATTGGCGGCGATGAAAC	TCGTCGGCTTGAATGGTTAC	50.0	50.0	58.0	58.3	126-130 bp	1.0	80.6
atpF-atpA_p4	CATATTGGCGGCGATGAAAC	TTTCGTCGGCTTGAATGGTT	50.0	45.0	58.0	58.4	128-132 bp	1.0	79.6
atpF-atpA_p5	CATATTGGCGGCGATGAAAC	ACTTGAAGTACGGTACCGGT	50.0	50.0	58.0	58.4	207-211 bp	1.0	78.6