Hotspot

atpH-atpF

LSC · 26827-27192 · 366 bp

Back to Verbesina

Pi 0.0329

MarkerSeek score 73.00

Primer available yes

Label atpH-atpF

Feature Metadata

label_name: atpH-atpF
feature_type: igs
region: LSC
length_bp: 366
variable_sites: 12
pi: 0.0329
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpF_p1	GCCTGGTTGTAGCATTAGCA	TGGCCCAAAGAAACGAAAGA	50.0	45.0	58.2	57.9	451-452 bp	1.0	79.8
atpH-atpF_p2	GCCTGGTTGTAGCATTAGCA	ATTAAACCCGAAACTCCCGG	50.0	50.0	58.2	57.9	482-483 bp	1.0	79.2
atpH-atpF_p3	GCCTGGTTGTAGCATTAGCA	GTGGCCCAAAGAAACGAAAG	50.0	50.0	58.2	57.9	452-453 bp	1.0	79.2
atpH-atpF_p4	TGGCCTGGTTGTAGCATTAG	TGGCCCAAAGAAACGAAAGA	50.0	45.0	57.6	57.9	453-454 bp	1.0	78.5
atpH-atpF_p5	GCCTGGTTGTAGCATTAGCA	AGTGGCCCAAAGAAACGAAA	50.0	45.0	58.2	58.2	453-454 bp	1.0	78.5