Hotspot

accD

LSC · 58395-60167 · 1773 bp

Back to Verbena

Pi 0.00509

MarkerSeek score 54.80

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1773
variable_sites: 9
pi: 0.00509
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	AAGGGGAGCAAAGGCATATG	TCCTTTCTTTGTGTTTTCTTCTTGT	50.0	32.0	57.9	57.8	1927-1975 bp	1.0	40.6
accD_p2	AAGGGGAGCAAAGGCATATG	CCTTTCTTTGTGTTTTCTTCTTGT	50.0	33.3	57.9	56.5	1926-1974 bp	1.0	40.6
accD_p3	AAGGGGAGCAAAGGCATATG	TCTTCCTTTCTTTGTGTTTTCTTCT	50.0	32.0	57.9	57.3	1930-1978 bp	1.0	40.6
accD_p4	GAGCAAAGGCATATGCAGGT	TCCTTTCTTTGTGTTTTCTTCTTGT	50.0	32.0	58.6	57.8	1922-1970 bp	1.0	40.6
accD_p5	AAGGGGAGCAAAGGCATATG	TCCTTTCTTTGTGTTTTCTTCTTG	50.0	33.3	57.9	56.2	1927-1975 bp	1.0	40.6