Hotspot

ndhE-ndhG

SSC · 117579-117805 · 227 bp

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Pi 0.00901

MarkerSeek score 59.80

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 227
variable_sites: 2
pi: 0.00901
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	GACACATAAGGGCTCTAACCA	GGTGCAATTGCTATAGCTCG	47.6	50.0	57.1	57.0	349-354 bp	1.0	58.7
ndhE-ndhG_p2	AGTTCAAGACACATAAGGGCTC	GGTGCAATTGCTATAGCTCG	45.5	50.0	58.1	57.0	356-361 bp	1.0	57.5
ndhE-ndhG_p3	GTTCAAGACACATAAGGGCTCT	GGTGCAATTGCTATAGCTCG	45.5	50.0	58.1	57.0	355-360 bp	1.0	57.5
ndhE-ndhG_p4	AGTTCAAGACACATAAGGGCT	GGTGCAATTGCTATAGCTCG	42.9	50.0	56.9	57.0	356-361 bp	1.0	57.3
ndhE-ndhG_p5	GACACATAAGGGCTCTAACCA	TGCAATTGCTATAGCTCGTCA	47.6	42.9	57.1	57.8	347-352 bp	1.0	56.9