Hotspot

rpl32-trnL-UAG

SSC · 112962-113771 · 810 bp

Back to Verbena

Pi 0.00865

MarkerSeek score 63.80

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 810
variable_sites: 7
pi: 0.00865
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AAAGGGAAGGGGTATAGGGC	TAGGAGATGATATGCCGCCA	55.0	50.0	58.5	58.1	1019-1033 bp	1.0	76.4
rpl32-trnL-UAG_p2	GGCAGCGTTAAAAGCGTTTT	TAGGAGATGATATGCCGCCA	45.0	50.0	58.5	58.1	1002-1016 bp	1.0	76.1
rpl32-trnL-UAG_p3	AAAGGGAAGGGGTATAGGGC	TCCTAAGAGCAGCGTGTCTA	55.0	50.0	58.5	57.9	966-980 bp	1.0	75.9
rpl32-trnL-UAG_p4	GGCAGCGTTAAAAGCGTTTT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	58.5	57.9	949-963 bp	1.0	75.5
rpl32-trnL-UAG_p5	AAAGGGAAGGGGTATAGGGC	AGCAGCGTGTCTACCAATTT	55.0	45.0	58.5	57.8	959-973 bp	1.0	75.4