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psbT-psbN

LSC · 74545-74609 · 65 bp

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Pi 0.0308

MarkerSeek score 60.10

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 65
variable_sites: 2
pi: 0.0308
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	CGAGAACCACCTAAAGTTCCA	GGGTACGCCTTATATACCGC	47.6	55.0	57.6	57.7	173 bp	1.0	67.5
psbT-psbN_p2	CGAGAACCACCTAAAGTTCCA	GATCCATTCGAGGAACACGG	47.6	55.0	57.6	58.4	125 bp	1.0	66.7
psbT-psbN_p3	CGAGAACCACCTAAAGTTCCA	AGATCCATTCGAGGAACACG	47.6	50.0	57.6	57.4	126 bp	1.0	65.4
psbT-psbN_p4	TCGAGAACCACCTAAAGTTCC	GGGTACGCCTTATATACCGC	47.6	55.0	57.3	57.7	174 bp	1.0	65.3
psbT-psbN_p5	TCGAGAACCACCTAAAGTTCC	GATCCATTCGAGGAACACGG	47.6	55.0	57.3	58.4	126 bp	1.0	64.5