Hotspot

ycf3-trnS-GGA

LSC · 45421-46300 · 880 bp

Back to Verbena

Pi 0.00796
MarkerSeek score 59.50
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
880
variable_sites
7
pi
0.00796
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 TGCCTCCTTTTCTCCTGAAG GCGAAGGTACGGAAAGAGAG 50.0 55.0 57.1 58.1 1080-1081 bp 1.0 72.9
ycf3-trnS-GGA_p2 GCCTCCTTTTCTCCTGAAGT GCGAAGGTACGGAAAGAGAG 50.0 55.0 57.1 58.1 1079-1080 bp 1.0 72.9
ycf3-trnS-GGA_p3 TGCCTCCTTTTCTCCTGAAG CCCTCGGTAAACAAAAGCCT 50.0 50.0 57.1 58.1 1051-1052 bp 1.0 72.9
ycf3-trnS-GGA_p4 GCCTCCTTTTCTCCTGAAGT CCCTCGGTAAACAAAAGCCT 50.0 50.0 57.1 58.1 1050-1051 bp 1.0 72.9
ycf3-trnS-GGA_p5 TGCCTCCTTTTCTCCTGAAG ACGGAAAGAGAGGGATTCGA 50.0 50.0 57.1 58.2 1072-1073 bp 1.0 72.4