Hotspot

psaA-ycf3

LSC · 42738-43475 · 738 bp

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Pi 0.00542

MarkerSeek score 60.50

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 738
variable_sites: 4
pi: 0.00542
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TTACTTTTGGTTCCGGCGAA	ATCAAGCCGCTGAGTATTGG	45.0	50.0	58.0	58.0	867-868 bp	1.0	79.8
psaA-ycf3_p2	TTTACTTTTGGTTCCGGCGA	ATCAAGCCGCTGAGTATTGG	45.0	50.0	58.0	58.0	868-869 bp	1.0	79.8
psaA-ycf3_p3	TTACTTTTGGTTCCGGCGAA	GGAAACAGGCTATAGCGCTT	45.0	50.0	58.0	58.0	849-850 bp	1.0	79.7
psaA-ycf3_p4	TTTACTTTTGGTTCCGGCGA	GGAAACAGGCTATAGCGCTT	45.0	50.0	58.0	58.0	850-851 bp	1.0	79.7
psaA-ycf3_p5	TGAGAAATGACCCGGTTTGG	ATCAAGCCGCTGAGTATTGG	50.0	50.0	58.1	58.0	935-936 bp	1.0	79.2