Hotspot

rpoB-trnC-GCA

LSC · 27678-28847 · 1170 bp

Back to Verbena

Pi 0.00769

MarkerSeek score 61.60

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1170
variable_sites: 9
pi: 0.00769
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CATTGCCTCATTTGCATCCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.8	58.1	1259-1260 bp	1.0	78.0
rpoB-trnC-GCA_p2	CATTGCCTCATTTGCATCCC	AAAAGGATTTGCAGTCCCCC	50.0	50.0	57.8	58.4	1241-1242 bp	1.0	75.9
rpoB-trnC-GCA_p3	CATTGCCTCATTTGCATCCC	TCTGCGCGTTCTTAGAAGAA	50.0	45.0	57.8	57.3	1317-1318 bp	1.0	72.9
rpoB-trnC-GCA_p4	ATTGCCTCATTTGCATCCCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.9	58.1	1258-1259 bp	1.0	72.9
rpoB-trnC-GCA_p5	CATTGCCTCATTTGCATCCC	TTTGTTGATCAGGCGACACC	50.0	50.0	57.8	58.8	1278-1279 bp	1.0	72.6