Hotspot

trnS-GCU-trnG-UCC

LSC · 9116-9821 · 706 bp

Back to Verbena

Pi 0.00725

MarkerSeek score 63.60

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 706
variable_sites: 5
pi: 0.00725
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	AGGGGTAATAGAACGAATCACAC	50.0	43.5	58.0	58.0	779-787 bp	1.0	58.0
trnS-GCU-trnG-UCC_p2	CAATCCGACGCTTTAGTCCA	GGGGTAATAGAACGAATCACACT	50.0	43.5	58.0	58.0	778-786 bp	1.0	58.0
trnS-GCU-trnG-UCC_p3	AATCCGACGCTTTAGTCCAC	AGGGGTAATAGAACGAATCACAC	50.0	43.5	58.0	58.0	778-786 bp	1.0	57.9
trnS-GCU-trnG-UCC_p4	AATCCGACGCTTTAGTCCAC	GGGGTAATAGAACGAATCACACT	50.0	43.5	58.0	58.0	777-785 bp	1.0	57.9
trnS-GCU-trnG-UCC_p5	ACGGAAAGAGAGGGATTCGA	GGGGTAATAGAACGAATCACACT	50.0	43.5	58.2	58.0	832-840 bp	1.0	56.6