Hotspot

trnK-UUU-rps16

LSC · 4213-5071 · 859 bp

Back to Verbena

Pi 0.00931

MarkerSeek score 64.10

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 859
variable_sites: 8
pi: 0.00931
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	GCTCAACCCACAGAAACTGT	50.0	50.0	58.2	58.3	980-985 bp	1.0	76.7
trnK-UUU-rps16_p2	AAAGCCGAGTACTCTACCGT	GTGCTCAACCCACAGAAACT	50.0	50.0	58.2	58.3	982-987 bp	1.0	76.7
trnK-UUU-rps16_p3	AAGCCGAGTACTCTACCGTT	GCTCAACCCACAGAAACTGT	50.0	50.0	58.2	58.3	979-984 bp	1.0	76.7
trnK-UUU-rps16_p4	AAGCCGAGTACTCTACCGTT	GTGCTCAACCCACAGAAACT	50.0	50.0	58.2	58.3	981-986 bp	1.0	76.7
trnK-UUU-rps16_p5	AAGCCGAGTACTCTACCGTT	TGCTCAACCCACAGAAACTG	50.0	50.0	58.2	58.3	980-985 bp	1.0	76.7