Hotspot

ndhD

SSC · 115378-116880 · 1503 bp

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Pi 0.002

MarkerSeek score 33.70

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 7
pi: 0.002
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 33.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	AGGATTCTTTTTGTGATTGTACA	ACGGATTTTTCTGGCCCAAG	30.4	50.0	54.5	58.7	1605 bp	1.0	40.3
ndhD_p2	AGGATTCTTTTTGTGATTGTACA	CGGATTTTTCTGGCCCAAGT	30.4	50.0	54.5	58.7	1604 bp	1.0	40.3
ndhD_p3	AGGATTCTTTTTGTGATTGTACA	GGATTTTTCTGGCCCAAGTG	30.4	50.0	54.5	57.3	1603 bp	1.0	40.3
ndhD_p4	AGGATTCTTTTTGTGATTGTACAA	ACGGATTTTTCTGGCCCAAG	29.2	50.0	55.2	58.7	1605 bp	1.0	40.3
ndhD_p5	AGGATTCTTTTTGTGATTGTACAA	CGGATTTTTCTGGCCCAAGT	29.2	50.0	55.2	58.7	1604 bp	1.0	40.3