Hotspot

atpF

LSC · 11856-13120 · 1265 bp

Back to Verbascum

Pi 0.003

MarkerSeek score 61.20

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1265
variable_sites: 9
pi: 0.003
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	TTTCATCGGCTCGAATGGTT	AGAGGGGCAAAGTGATAGAA	45.0	45.0	57.9	55.8	1409-1415 bp	1.0	62.5
atpF_p2	TTTCATCGGCTCGAATGGTT	AAGAGGGGCAAAGTGATAGA	45.0	45.0	57.9	55.8	1410-1416 bp	1.0	62.4
atpF_p3	TCATCGGCTCGAATGGTTAC	AAGAGGGGCAAAGTGATAGA	50.0	45.0	57.8	55.8	1408-1414 bp	1.0	61.6
atpF_p4	TCATCGGCTCGAATGGTTAC	AGAGGGGCAAAGTGATAGAA	50.0	45.0	57.8	55.8	1407-1413 bp	1.0	61.6
atpF_p5	TTTCATCGGCTCGAATGGTT	AGAGGGGCAAAGTGATAGAAA	45.0	42.9	57.9	56.6	1409-1415 bp	1.0	60.8