Hotspot

ndhD-psaC

SSC · 116881-117004 · 124 bp

Back to Verbascum

Pi 0.0468

MarkerSeek score 68.60

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 124
variable_sites: 10
pi: 0.0468
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	GAACCCGCAAATATTGGCAA	ATGGTCTGAAACAACTCGCA	45.0	45.0	57.3	57.7	220-221 bp	1.0	76.3
ndhD-psaC_p2	GAACCCGCAAATATTGGCAA	AAGAGATGTGAATCCGCCTG	45.0	50.0	57.3	57.7	273-274 bp	1.0	75.0
ndhD-psaC_p3	GAACCCGCAAATATTGGCAA	GGTCTGAAACAACTCGCAGT	45.0	50.0	57.3	58.4	218-219 bp	1.0	75.0
ndhD-psaC_p4	GAACCCGCAAATATTGGCAA	TGGTCTGAAACAACTCGCAG	45.0	50.0	57.3	58.4	219-220 bp	1.0	75.0
ndhD-psaC_p5	GAACCCGCAAATATTGGCAA	GAGATGTGAATCCGCCTGTC	45.0	55.0	57.3	58.4	271-272 bp	1.0	74.2