Hotspot

accD-psaI

LSC · 59780-60238 · 459 bp

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Pi 0.00523

MarkerSeek score 61.60

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 459
variable_sites: 6
pi: 0.00523
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	CCAGAAGGGCTTATTCGACC	TTGCAAGTGCCGGAAATACT	55.0	45.0	58.0	58.1	633-862 bp	1.0	80.4
accD-psaI_p2	CCAGAAGGGCTTATTCGACC	AAAGAAGCCATTGCAAGTGC	55.0	45.0	58.0	57.8	643-872 bp	1.0	79.9
accD-psaI_p3	TTCCACGCCTTCTTTCCTTT	TTGCAAGTGCCGGAAATACT	45.0	45.0	57.6	58.1	563-792 bp	1.0	78.1
accD-psaI_p4	AAGTTCCACGCCTTCTTTCC	TTGCAAGTGCCGGAAATACT	50.0	45.0	58.4	58.1	566-795 bp	1.0	77.8
accD-psaI_p5	GTTCCACGCCTTCTTTCCTT	TTGCAAGTGCCGGAAATACT	50.0	45.0	58.4	58.1	564-793 bp	1.0	77.8