Hotspot

psaA-ycf3

LSC · 42206-43018 · 813 bp

Back to Verbascum

Pi 0.00584

MarkerSeek score 65.70

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 813
variable_sites: 10
pi: 0.00584
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TGAGAAATGACCCGGTTTGG	GGAAACAGGCTATAGCGCTT	50.0	50.0	58.1	58.0	952-1045 bp	1.0	83.7
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	GGAAACAGGCTATAGCGCTT	50.0	50.0	57.9	58.0	933-1026 bp	1.0	83.5
psaA-ycf3_p3	TGAGAAATGACCCGGTTTGG	GAAAATCACGAGGCGTTTCG	50.0	50.0	58.1	58.1	897-990 bp	1.0	83.0
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	GAAAATCACGAGGCGTTTCG	50.0	50.0	57.9	58.1	878-971 bp	1.0	82.9
psaA-ycf3_p5	TGAGAAATGACCCGGTTTGG	AATCACGAGGCGTTTCGAAT	50.0	45.0	58.1	58.3	894-987 bp	1.0	81.9