Hotspot

atpH-atpI

LSC · 13717-14765 · 1049 bp

Back to Verbascum

Pi 0.00427

MarkerSeek score 64.50

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1049
variable_sites: 11
pi: 0.00427
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TCCCTGTCATGTTCCTTGGA	45.0	50.0	58.0	58.3	1159-1185 bp	1.0	80.2
atpH-atpI_p2	AATAACGGAAGCGGCAGAAA	TCCCTGTCATGTTCCTTGGA	45.0	50.0	58.2	58.3	1160-1186 bp	1.0	79.1
atpH-atpI_p3	GCCAATCCAGCAGCAATAAC	TCCCTGTCATGTTCCTTGGA	50.0	50.0	57.8	58.3	1174-1200 bp	1.0	78.8
atpH-atpI_p4	TCCAGCAGCAATAACGGAAG	TCCCTGTCATGTTCCTTGGA	50.0	50.0	58.3	58.3	1169-1195 bp	1.0	78.4
atpH-atpI_p5	ATAACGGAAGCGGCAGAAAT	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.0	58.5	1111-1137 bp	1.0	78.3