Hotspot

rpl16

LSC · 80255-81630 · 1376 bp

Back to Veratrum

Pi 0.0165

MarkerSeek score 51.70

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1376
variable_sites: 55
pi: 0.0165
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	ATCAATTTTCGAGCTCCGCT	GATCAGAGAGGGTAGGGTCC	45.0	60.0	58.0	58.0	1843-1856 bp	1.0	83.7
rpl16_p2	ATCAATTTTCGAGCTCCGCT	GGATCAGAGAGGGTAGGGTC	45.0	60.0	58.0	58.0	1844-1857 bp	1.0	83.7
rpl16_p3	CGCACCAAAAACTCGAGTTC	GATCAGAGAGGGTAGGGTCC	50.0	60.0	58.0	58.0	2087-2100 bp	1.0	83.4
rpl16_p4	CGCACCAAAAACTCGAGTTC	GGATCAGAGAGGGTAGGGTC	50.0	60.0	58.0	58.0	2088-2101 bp	1.0	83.4
rpl16_p5	CGCACCAAAAACTCGAGTTC	GGTTTCGAAGGAATTGCACG	50.0	50.0	58.0	58.0	2455-2468 bp	0.7	70.1