Hotspot

rpl32-ndhF

SSC · 124571-125093 · 523 bp

Back to Veratrum

Pi 0.026

MarkerSeek score 57.60

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 523
variable_sites: 33
pi: 0.026
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	CCCTTCTTCTTCCAAATATTTTTACG	TGCATATTGATATGTATGTTCCA	34.6	30.4	57.1	53.7	521-620 bp	1.0	48.7
rpl32-ndhF_p2	TCCCTTCTTCTTCCAAATATT	TGCATATTGATATGTATGTTCCA	33.3	30.4	52.1	53.7	522-621 bp	1.0	48.7
rpl32-ndhF_p3	TCCCTTCTTCTTCCAAATATTT	TGCATATTGATATGTATGTTCCA	31.8	30.4	53.0	53.7	522-621 bp	1.0	48.7
rpl32-ndhF_p4	CCCTTCTTCTTCCAAATATTTTTACG	TCAATGCATATTGATATGTATGTTCCA	34.6	29.6	57.1	57.5	525-624 bp	1.0	48.7
rpl32-ndhF_p5	TCCCTTCTTCTTCCAAATATT	TCAATGCATATTGATATGTATGTTCCA	33.3	29.6	52.1	57.5	526-625 bp	1.0	48.7