Hotspot

trnL-UAG-rpl32

SSC · 123760-124396 · 637 bp

Back to Veratrum

Pi 0.0237
MarkerSeek score 62.20
Primer available yes
Label trnL-UAG-rpl32

Feature Metadata

label_name
trnL-UAG-rpl32
feature_type
igs
region
SSC
length_bp
637
variable_sites
34
pi
0.0237
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.96
markerseek_score
62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-rpl32_p1 AGCGTGTCTACCGATTTCAC CCACAGGGCATTCAAAGAGT 50.0 50.0 58.0 58.1 707-725 bp 0.9 82.1
trnL-UAG-rpl32_p2 CAGCGTGTCTACCGATTTCA CCACAGGGCATTCAAAGAGT 50.0 50.0 58.0 58.1 708-726 bp 0.9 82.1
trnL-UAG-rpl32_p3 TCCTAAGAGCAGCGTGTCTA CCACAGGGCATTCAAAGAGT 50.0 50.0 57.9 58.1 717-735 bp 0.9 81.1
trnL-UAG-rpl32_p4 AGCGTGTCTACCGATTTCAC TCCACAGGGCATTCAAAGAG 50.0 50.0 58.0 57.8 708-726 bp 0.9 81.1
trnL-UAG-rpl32_p5 CAGCGTGTCTACCGATTTCA TCCACAGGGCATTCAAAGAG 50.0 50.0 58.0 57.8 709-727 bp 0.9 81.1