Hotspot

trnP-UGG-psaJ

LSC · 65927-66339 · 413 bp

Back to Veratrum

Pi 0.0332

MarkerSeek score 59.30

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 413
variable_sites: 36
pi: 0.0332
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	AAAACAAACGCGCTACCAAG	TGGAGAGATATGTTTTTAGATATTGCA	45.0	29.6	57.9	56.9	456-490 bp	1.0	50.1
trnP-UGG-psaJ_p2	CAAAACAAACGCGCTACCAA	TGGAGAGATATGTTTTTAGATATTGCA	45.0	29.6	58.2	56.9	457-491 bp	1.0	50.1
trnP-UGG-psaJ_p3	AAAACAAACGCGCTACCAAG	GTGGAGAGATATGTTTTTAGATATTGC	45.0	33.3	57.9	56.7	457-491 bp	1.0	50.1
trnP-UGG-psaJ_p4	TACCCAAAACAAACGCGCTA	TGGAGAGATATGTTTTTAGATATTGCA	45.0	29.6	58.4	56.9	461-495 bp	1.0	50.1
trnP-UGG-psaJ_p5	TTGTACCCAAAACAAACGCG	TGGAGAGATATGTTTTTAGATATTGCA	45.0	29.6	57.8	56.9	464-498 bp	1.0	50.0