Hotspot

ycf4-cemA

LSC · 59455-60166 · 712 bp

Back to Veratrum

Pi 0.0231

MarkerSeek score 60.30

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 712
variable_sites: 39
pi: 0.0231
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	GCGCGTACCGATTGAAGTAT	TGGGAAGGAAGTTAATGTTT	50.0	35.0	58.5	52.2	750-773 bp	1.0	47.4
ycf4-cemA_p2	GCGCGTACCGATTGAAGTAT	TGGGAAGGAAGTTAATGTTTT	50.0	33.3	58.5	53.2	750-773 bp	1.0	47.4
ycf4-cemA_p3	GCGCGTACCGATTGAAGTAT	ACAAAATATGGGAAGGAAGTT	50.0	33.3	58.5	52.9	758-781 bp	1.0	47.4
ycf4-cemA_p4	GCGCGTACCGATTGAAGTAT	ATGGGAAGGAAGTTAATGTTT	50.0	33.3	58.5	52.9	751-774 bp	1.0	47.4
ycf4-cemA_p5	GCGCGTACCGATTGAAGTAT	ATGGGAAGGAAGTTAATGTTTT	50.0	31.8	58.5	53.8	751-774 bp	1.0	47.4