Hotspot

ndhC-trnV-UAC

LSC · 49446-50669 · 1224 bp

Back to Veratrum

Pi 0.0199
MarkerSeek score 55.80
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1224
variable_sites
57
pi
0.0199
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
55.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 AGGTGCTAAAACTCCGGAAA AGAAGGTCTACGGTTCGAGT 45.0 50.0 57.1 57.8 1204-1380 bp 1.0 78.3
ndhC-trnV-UAC_p2 AGGTGCTAAAACTCCGGAAA GAAGGTCTACGGTTCGAGTC 45.0 55.0 57.1 57.5 1203-1379 bp 1.0 75.6
ndhC-trnV-UAC_p3 AGGTGCTAAAACTCCGGAAA GTTCGAGTCCGTATAGCCCT 45.0 55.0 57.1 58.7 1192-1368 bp 1.0 74.5
ndhC-trnV-UAC_p4 AGGTGCTAAAACTCCGGAAAT AGAAGGTCTACGGTTCGAGT 42.9 50.0 57.6 57.8 1204-1380 bp 1.0 74.4
ndhC-trnV-UAC_p5 AGGTGCTAAAACTCCGGAAA AAGGTCTACGGTTCGAGTCC 45.0 55.0 57.1 58.8 1202-1378 bp 1.0 73.3