Pi
0.0283
MarkerSeek score
74.00
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | AGAGAAAAAGCCGGCTATCG | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.1 | 58.0 | 850-866 bp | 1.0 | 87.0 |
| trnT-UGU-trnL-UAA_p2 | CGATAGAGAAAAAGCCGGCT | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.1 | 58.0 | 854-870 bp | 1.0 | 86.9 |
| trnT-UGU-trnL-UAA_p3 | TCGATAGAGAAAAAGCCGGC | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.1 | 58.0 | 855-871 bp | 1.0 | 86.9 |
| trnT-UGU-trnL-UAA_p4 | AGAGAAAAAGCCGGCTATCG | TTTCCATGCCAAGGCTCAAT | 50.0 | 45.0 | 58.1 | 58.1 | 891-907 bp | 1.0 | 86.2 |
| trnT-UGU-trnL-UAA_p5 | CGATAGAGAAAAAGCCGGCT | TTTCCATGCCAAGGCTCAAT | 50.0 | 45.0 | 58.1 | 58.1 | 895-911 bp | 1.0 | 86.2 |