Hotspot

trnT-GGU-psbD

LSC · 30910-31732 · 823 bp

Back to Veratrum

Pi 0.0213

MarkerSeek score 54.70

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 823
variable_sites: 42
pi: 0.0213
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 54.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	AAGCACCATGAAGAGCAACA	55.0	45.0	58.1	58.0	1226-1242 bp	1.0	84.2
trnT-GGU-psbD_p2	GTGGTAGAGTAACGCCATGG	AGCACCATGAAGAGCAACAA	55.0	45.0	58.1	58.0	1225-1241 bp	1.0	84.2
trnT-GGU-psbD_p3	GTGGTAGAGTAACGCCATGG	AGGACGCAATTGGACAGATC	55.0	50.0	58.1	58.0	1288-1304 bp	1.0	83.9
trnT-GGU-psbD_p4	GTGGTAGAGTAACGCCATGG	ACAAAAACAGCAATTGGGCC	55.0	45.0	58.1	58.0	1329-1345 bp	1.0	83.7
trnT-GGU-psbD_p5	GTGGTAGAGTAACGCCATGG	CTGCTACACCAAAACTGGGT	55.0	50.0	58.1	58.0	1397-1413 bp	0.7	71.3