Hotspot

atpH-atpI

LSC · 13431-14186 · 756 bp

Back to Veratrum

Pi 0.0182

MarkerSeek score 59.20

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 756
variable_sites: 32
pi: 0.0182
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ACGGAAGCGGCAGAAATTAT	CGAATCCATGGAGGGTCATC	45.0	55.0	58.0	57.8	810-821 bp	0.8	73.6
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	CGAATCCATGGAGGGTCATC	45.0	55.0	58.0	57.8	813-824 bp	0.8	73.6
atpH-atpI_p3	TACCTTCTACAGCTTGGCCT	CGAATCCATGGAGGGTCATC	50.0	55.0	58.0	57.8	896-907 bp	0.8	73.2
atpH-atpI_p4	AACGGAAGCGGCAGAAATTA	CGAATCCATGGAGGGTCATC	45.0	55.0	58.2	57.8	811-822 bp	0.8	72.5
atpH-atpI_p5	TAACGGAAGCGGCAGAAATT	CGAATCCATGGAGGGTCATC	45.0	55.0	58.2	57.8	812-823 bp	0.8	72.5