Hotspot

trnS-GCU-trnG-UCC

LSC · 7782-8695 · 914 bp

Back to Veratrum

Pi 0.0263

MarkerSeek score 62.10

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 914
variable_sites: 55
pi: 0.0263
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	ATTAGCAATCCGCCGCTTTA	ACGAATCACACTTTTACCACT	45.0	38.1	58.0	55.5	925-988 bp	1.0	60.0
trnS-GCU-trnG-UCC_p2	ATTAGCAATCCGCCGCTTTA	AACGAATCACACTTTTACCACT	45.0	36.4	58.0	56.2	926-989 bp	1.0	58.1
trnS-GCU-trnG-UCC_p3	GCTTTAGTCCACTCAGCCAT	ACGAATCACACTTTTACCACT	50.0	38.1	57.6	55.5	911-974 bp	1.0	57.1
trnS-GCU-trnG-UCC_p4	ATTAGCAATCCGCCGCTTTA	TACCACTAAACTATACCCGC	45.0	45.0	58.0	54.0	911-974 bp	1.0	56.3
trnS-GCU-trnG-UCC_p5	AGTCCACTCAGCCATCTCTC	ACGAATCACACTTTTACCACT	55.0	38.1	58.5	55.5	906-969 bp	1.0	56.3