Hotspot

psbK-psbI

LSC · 6982-7458 · 477 bp

Back to Veratrum

Pi 0.0291

MarkerSeek score 55.20

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 477
variable_sites: 34
pi: 0.0291
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 55.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	ACGAATAGTTTGAGAGTAAGCA	50.0	36.4	57.9	55.2	444-531 bp	1.0	51.2
psbK-psbI_p2	TGGCAAGCTGCTGTAAGTTT	ACGAATAGTTTGAGAGTAAGCA	45.0	36.4	58.3	55.2	441-528 bp	1.0	49.8
psbK-psbI_p3	TTGGCAAGCTGCTGTAAGTT	ACGAATAGTTTGAGAGTAAGCA	45.0	36.4	58.3	55.2	442-529 bp	1.0	49.8
psbK-psbI_p4	TTTGGCAAGCTGCTGTAAGT	ACGAATAGTTTGAGAGTAAGCA	45.0	36.4	58.3	55.2	443-530 bp	1.0	49.8
psbK-psbI_p5	GTTTGGCAAGCTGCTGTAAG	AACGAATAGTTTGAGAGTAAGCA	50.0	34.8	57.9	56.0	445-532 bp	1.0	49.7