Hotspot

trnK-UUU-trnQ-UUG

LSC · 4020-6380 · 2361 bp

Back to Veratrum

Pi 0.0156

MarkerSeek score 43.40

Primer available yes

Label trnK-UUU-trnQ-UUG

Feature Metadata

label_name: trnK-UUU-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 2361
variable_sites: 80
pi: 0.0156
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.47
markerseek_score: 43.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-trnQ-UUG_p1	CCTTTCAGGATCAGTCGTGG	TTTGGGCTGGAATTGACGAA	55.0	45.0	58.0	58.0	1374-2631 bp	1.0	87.5
trnK-UUU-trnQ-UUG_p2	TCAGGATCAGTCGTGGTCTT	TTTGGGCTGGAATTGACGAA	50.0	45.0	58.1	58.0	1370-2627 bp	1.0	87.1
trnK-UUU-trnQ-UUG_p3	TTCAGGATCAGTCGTGGTCT	TTTGGGCTGGAATTGACGAA	50.0	45.0	58.1	58.0	1371-2628 bp	1.0	87.1
trnK-UUU-trnQ-UUG_p4	CTGTCCCCTTCCAAAAACCA	TTTGGGCTGGAATTGACGAA	50.0	45.0	57.9	58.0	1526-2784 bp	1.0	86.9
trnK-UUU-trnQ-UUG_p5	CCTTTCAGGATCAGTCGTGG	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.0	58.1	1268-2516 bp	0.8	78.5