Hotspot

rpl32-trnL-UAG

SSC · 116700-117745 · 1046 bp

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Pi 0.0153
MarkerSeek score 58.00
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1046
variable_sites
60
pi
0.0153
unique_haplotype_count
11
primer_available
yes
alignment_reliability
0.99
markerseek_score
58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 ACAAATAAATAAGAAAACGTTGGA AGCGTGTCTACCGATTTCAC 25.0 50.0 53.7 58.0 1092-1121 bp 1.0 47.0
rpl32-trnL-UAG_p2 ACAAATAAATAAGAAAACGTTGGA CAGCGTGTCTACCGATTTCA 25.0 50.0 53.7 58.0 1093-1122 bp 1.0 47.0
rpl32-trnL-UAG_p3 ACAAATAAATAAGAAAACGTTGGA TCCTAAGAGCAGCGTGTCTA 25.0 50.0 53.7 57.9 1102-1131 bp 1.0 46.9
rpl32-trnL-UAG_p4 ACAAATAAATAAGAAAACGTTGGA CCTAAGAGCAGCGTGTCTAC 25.0 55.0 53.7 57.8 1101-1130 bp 1.0 46.9
rpl32-trnL-UAG_p5 ACAAATAAATAAGAAAACGTTGGA CTAAGAGCAGCGTGTCTACC 25.0 55.0 53.7 57.8 1100-1129 bp 1.0 46.9