Hotspot

petA-psbJ

LSC · 65025-66112 · 1088 bp

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Pi 0.0112
MarkerSeek score 62.90
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1088
variable_sites
50
pi
0.0112
unique_haplotype_count
12
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 ATCCATTACGTGTCCAAGGC CCGGATTAGGTTCGTCCTTG 50.0 55.0 58.0 58.1 1221-1240 bp 1.0 85.8
petA-psbJ_p2 ATCCATTACGTGTCCAAGGC TCCGGATTAGGTTCGTCCTT 50.0 50.0 58.0 58.1 1222-1241 bp 1.0 85.2
petA-psbJ_p3 CATTACGTGTCCAAGGCCTT CCGGATTAGGTTCGTCCTTG 50.0 55.0 58.2 58.1 1218-1237 bp 1.0 84.7
petA-psbJ_p4 GCATCTGTTGTTTTGGCACA CCGGATTAGGTTCGTCCTTG 45.0 55.0 57.8 58.1 1186-1205 bp 1.0 84.6
petA-psbJ_p5 TTGGCATCTGTTGTTTTGGC CCGGATTAGGTTCGTCCTTG 45.0 55.0 57.8 58.1 1189-1208 bp 1.0 84.4