Hotspot

psaI-ycf4

LSC · 61632-62071 · 440 bp

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Pi 0.0124
MarkerSeek score 68.20
Primer available yes
Label psaI-ycf4

Feature Metadata

label_name
psaI-ycf4
feature_type
igs
region
LSC
length_bp
440
variable_sites
23
pi
0.0124
unique_haplotype_count
12
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaI-ycf4_p1 GCAATGGCAATGGCTTCTTT AAAAAGGATAAGGGCCCAGC 45.0 50.0 57.9 58.1 570-619 bp 1.0 83.0
psaI-ycf4_p2 GCAATGGCAATGGCTTCTTT TAAGGGCCCAGCAGAAATTG 45.0 50.0 57.9 58.1 562-611 bp 1.0 83.0
psaI-ycf4_p3 AGTATTTCCGGCAATGGCAA AAAAAGGATAAGGGCCCAGC 45.0 50.0 58.2 58.1 580-629 bp 1.0 82.5
psaI-ycf4_p4 AGTATTTCCGGCAATGGCAA TAAGGGCCCAGCAGAAATTG 45.0 50.0 58.2 58.1 572-621 bp 1.0 82.5
psaI-ycf4_p5 GGCCTAGTATTTCCGGCAAT AAAAAGGATAAGGGCCCAGC 50.0 50.0 57.7 58.1 585-634 bp 1.0 81.6