Hotspot

accD-psaI

LSC · 60763-61517 · 755 bp

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Pi 0.0169
MarkerSeek score 69.40
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
755
variable_sites
46
pi
0.0169
unique_haplotype_count
11
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 AAAAGGTGTTCTGAGCGAGT AAAGAAGCCATTGCCATTGC 45.0 45.0 57.4 57.9 862-909 bp 1.0 82.6
accD-psaI_p2 AAAGGTGTTCTGAGCGAGTT AAAGAAGCCATTGCCATTGC 45.0 45.0 57.4 57.9 861-908 bp 1.0 82.6
accD-psaI_p3 AAAAGGTGTTCTGAGCGAGT TTGCCATTGCCGGAAATACT 45.0 45.0 57.4 58.2 852-899 bp 1.0 82.2
accD-psaI_p4 AAAGGTGTTCTGAGCGAGTT TTGCCATTGCCGGAAATACT 45.0 45.0 57.4 58.2 851-898 bp 1.0 82.2
accD-psaI_p5 AAAAGGTGTTCTGAGCGAGTT AAAGAAGCCATTGCCATTGC 42.9 45.0 58.1 57.9 862-909 bp 1.0 78.9