Hotspot

atpA-atpF

LSC · 12098-12159 · 62 bp

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Pi 0.0516
MarkerSeek score 68.10
Primer available yes
Label atpA-atpF

Feature Metadata

label_name
atpA-atpF
feature_type
igs
region
LSC
length_bp
62
variable_sites
8
pi
0.0516
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpA-atpF_p1 AGCCTCGCTTACTGGTATCT CGGCCGCAAGATTCATTTTT 50.0 45.0 57.9 58.0 978-990 bp 0.923077 79.1
atpA-atpF_p2 CCGTTTCGGCTACCACAATA CGGCCGCAAGATTCATTTTT 50.0 45.0 58.0 58.0 1354-1366 bp 0.923077 79.0
atpA-atpF_p3 AGCCTCGCTTACTGGTATCT TGTTTGGGGCGATGAAAGAA 50.0 45.0 57.9 58.0 402-405 bp 0.923077 79.0
atpA-atpF_p4 CCGTTTCGGCTACCACAATA TGTTTGGGGCGATGAAAGAA 50.0 45.0 58.0 58.0 778-781 bp 0.923077 78.5
atpA-atpF_p5 CCGTTTCGGCTACCACAATA AGTCCTCCGAATATTCCGGT 50.0 50.0 58.0 57.9 1388-1400 bp 0.923077 78.4