Hotspot

trnR-UCU-atpA

LSC · 10350-10573 · 224 bp

Back to Vepris

Pi 0.0246
MarkerSeek score 57.50
Primer available yes
Label trnR-UCU-atpA

Feature Metadata

label_name
trnR-UCU-atpA
feature_type
igs
region
LSC
length_bp
224
variable_sites
17
pi
0.0246
unique_haplotype_count
11
primer_available
yes
alignment_reliability
0.92
markerseek_score
57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-atpA_p1 CTGAAAGGCGTCCATTGTCT TCAGGAACAAATGGAACGCT 50.0 45.0 58.2 57.7 317-394 bp 0.923077 81.1
trnR-UCU-atpA_p2 ACTGAAAGGCGTCCATTGTC TCAGGAACAAATGGAACGCT 50.0 45.0 58.5 57.7 318-395 bp 0.923077 78.8
trnR-UCU-atpA_p3 GACTGAAAGGCGTCCATTGT TCAGGAACAAATGGAACGCT 50.0 45.0 58.5 57.7 319-396 bp 0.923077 78.8
trnR-UCU-atpA_p4 TTCGGAATGGGACTGAAAGG TCAGGAACAAATGGAACGCT 50.0 45.0 57.5 57.7 329-406 bp 0.923077 78.8
trnR-UCU-atpA_p5 CTGAAAGGCGTCCATTGTCT TGGAACGCTTTCTACTTCAGG 50.0 47.6 58.2 58.0 306-383 bp 0.923077 75.1