Hotspot

trnS-GCU-trnT-CGU

LSC · 8310-9293 · 984 bp

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Pi 0.0127
MarkerSeek score 63.60
Primer available yes
Label trnS-GCU-trnT-CGU

Feature Metadata

label_name
trnS-GCU-trnT-CGU
feature_type
igs
region
LSC
length_bp
984
variable_sites
47
pi
0.0127
unique_haplotype_count
12
primer_available
yes
alignment_reliability
0.99
markerseek_score
63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnT-CGU_p1 CCGACGCTTTAATCCACTCA ACGAATCGCACTTTTACCACT 50.0 42.9 58.0 58.2 1049-1093 bp 0.923077 72.5
trnS-GCU-trnT-CGU_p2 CCGACGCTTTAATCCACTCA ACGAATCGCACTTTTACCAC 50.0 45.0 58.0 56.8 1049-1093 bp 0.923077 72.4
trnS-GCU-trnT-CGU_p3 ACGGAAAGAGAGGGATTCGA ACGAATCGCACTTTTACCACT 50.0 42.9 58.2 58.2 1107-1151 bp 0.923077 71.0
trnS-GCU-trnT-CGU_p4 ACGGAAAGAGAGGGATTCGA ACGAATCGCACTTTTACCAC 50.0 45.0 58.2 56.8 1107-1151 bp 0.923077 71.0
trnS-GCU-trnT-CGU_p5 ACGCTTTAATCCACTCAGCC ACGAATCGCACTTTTACCACT 50.0 42.9 58.3 58.2 1046-1090 bp 0.923077 70.5