Hotspot

ndhA

SSC · 127077-129711 · 2635 bp

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Pi 0.0171

MarkerSeek score 56.00

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2635
variable_sites: 40
pi: 0.0171
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 56.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	CTTGTTTGAGCCAAGTTGTGA	TGGGAGAAGTTGATCGTTGA	42.9	45.0	57.2	56.5	2406-2692 bp	1.0	58.9
ndhA_p2	TCTTGTTTGAGCCAAGTTGTG	TGGGAGAAGTTGATCGTTGA	42.9	45.0	57.2	56.5	2407-2693 bp	1.0	58.9
ndhA_p3	TGTTTGAGCCAAGTTGTGAA	TGGGAGAAGTTGATCGTTGA	40.0	45.0	56.0	56.5	2404-2690 bp	1.0	57.2
ndhA_p4	TTGTTTGAGCCAAGTTGTGA	TGGGAGAAGTTGATCGTTGA	40.0	45.0	56.0	56.5	2405-2691 bp	1.0	57.2
ndhA_p5	CTTGTTTGAGCCAAGTTGTGA	TGGGAGAAGTTGATCGTTGAA	42.9	42.9	57.2	57.2	2406-2692 bp	1.0	56.9