Hotspot

rpl32-trnL-UAG

SSC · 120245-121445 · 1201 bp

Back to Ventilago

Pi 0.0172

MarkerSeek score 71.30

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1201
variable_sites: 19
pi: 0.0172
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 71.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGGATATTGGGCAGCATTGA	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.0	58.0	1327-1587 bp	1.0	85.0
rpl32-trnL-UAG_p2	GGGATATTGGGCAGCATTGA	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.0	58.0	1328-1588 bp	1.0	85.0
rpl32-trnL-UAG_p3	AGGGATATTGGGCAGCATTG	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.0	58.0	1328-1588 bp	1.0	84.9
rpl32-trnL-UAG_p4	AGGGATATTGGGCAGCATTG	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.0	58.0	1329-1589 bp	1.0	84.9
rpl32-trnL-UAG_p5	GGGATATTGGGCAGCATTGA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	1337-1597 bp	1.0	84.0