Hotspot

ndhF-rpl32

SSC · 119363-120079 · 717 bp

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Pi 0.12

MarkerSeek score 84.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 717
variable_sites: 53
pi: 0.12
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.62
markerseek_score: 84.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TATTGCCGTCGGAAAAAGCA	TCAATGCTGCCCAATATCCC	45.0	50.0	58.5	58.0	628-904 bp	1.0	85.7
ndhF-rpl32_p2	TATTGCCGTCGGAAAAAGCA	CAATGCTGCCCAATATCCCT	45.0	50.0	58.5	58.0	627-903 bp	1.0	85.7
ndhF-rpl32_p3	CGTCGGAAAAAGCAGAAGTC	TCAATGCTGCCCAATATCCC	50.0	50.0	57.4	58.0	622-898 bp	1.0	84.9
ndhF-rpl32_p4	CGTCGGAAAAAGCAGAAGTC	CAATGCTGCCCAATATCCCT	50.0	50.0	57.4	58.0	621-897 bp	1.0	84.9
ndhF-rpl32_p5	TTATTGCCGTCGGAAAAAGC	TCAATGCTGCCCAATATCCC	45.0	50.0	57.4	58.0	629-905 bp	1.0	84.5