Hotspot

rps3-rpl22

LSC · 89811-89979 · 169 bp

Back to Ventilago

Pi 0.0741

MarkerSeek score 72.40

Primer available yes

Label rps3-rpl22

Feature Metadata

label_name: rps3-rpl22
feature_type: igs
region: LSC
length_bp: 169
variable_sites: 10
pi: 0.0741
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rpl22_p1	TGTGCCAAGTCTGAAACCAA	GGGGACGGGGTTATCCAATA	45.0	55.0	57.9	58.6	266-300 bp	1.0	81.2
rps3-rpl22_p2	TTGTGCCAAGTCTGAAACCA	GGGGACGGGGTTATCCAATA	45.0	55.0	57.9	58.6	267-301 bp	1.0	81.2
rps3-rpl22_p3	TGCCAAGTCTGAAACCAAGT	GGGGACGGGGTTATCCAATA	45.0	55.0	57.6	58.6	264-298 bp	1.0	78.9
rps3-rpl22_p4	ATGATGACCTTGGGTTGTGC	GGGGACGGGGTTATCCAATA	50.0	55.0	58.5	58.6	281-315 bp	1.0	78.6
rps3-rpl22_p5	GTTGTGCCAAGTCTGAAACC	GGGGACGGGGTTATCCAATA	50.0	55.0	57.5	58.6	268-302 bp	1.0	78.4